Science & Justice
Volume 47, Issue 4 , Pages 160-167 , December 2007

A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples

  • J.E. Allard

      Affiliations

    • Forensic Science Service, 109 Lambeth Road, London SE1 7LP, United Kingdom
    • Corresponding Author InformationCorresponding author.
    • ,
  • A. Baird

      Affiliations

    • Forensic Science Northern Ireland, 151 Belfast Road, Carrickfergus, Co. Antrim, BT38 8PL, Northern Ireland
    • ,
  • G. Davidson

      Affiliations

    • LGC Forensics Alliance, Birchwood Park, Darwin House, Faraday Road, Risley, Warrington, WA3 6AT, United Kingdom
    • Now at Forensic Science Service Chorley.
    • ,
  • S. Jones

      Affiliations

    • Grampian Police Forensic Laboratory, Nelson Street, Aberdeen, AB24 5EQ, United Kingdom
    • New Scottish Police Services Authority.
    • ,
  • J. Lewis

      Affiliations

    • LGC Forensics Alliance, Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom
    • Now at Cellmark Forensic Services, Abingdon.
    • ,
  • L. McKenna

      Affiliations

    • Forensic Science Laboratory, Garda Headquarters, Phoenix Park, Dublin 8, Ireland
    • ,
  • C. Weston

      Affiliations

    • Strathclyde Police Forensic Support Department, Police HQ, 173 Pitt St, Glasgow G2 4JS, United Kingdom
    • New Scottish Police Services Authority.
    • ,
  • D. Scrimger

      Affiliations

    • Lothian and Borders Police Forensic Science Laboratory, 11 Howden Hall Road, Edinburgh, EH16 6TL, United Kingdom
    • New Scottish Police Services Authority.
    • ,
  • G. Teppett

      Affiliations

    • Police Forensic Science Laboratory Dundee Tayside Police HQ, West Bell St, Dundee DD1 9JU, United Kingdom
    • New Scottish Police Services Authority.

,Accepted 26 September 2007.

References 

  1. Kind SS. The Acid Phosphatase Test. In:  Curry A editors. Methods of Forensic Science. London: Interscience; 1964;p. 267–288
  2. Biology Methods manual. Metropolitan Police Forensic Science Laboratory; 1978;
  3. Florence A. Du sperme et des taches de sperme en medicine legale. Archives d'Anthropologie Criminelle et de Psychologie Normale et Pathologique. 1896;11:37–46
  4. Forbes G. The scope and fallacies of the Florence reaction for seminal stains. The Police Journal. 1940;13:162–171
  5. Laurell CB. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry. 1966;15:45–52
  6. Sensabaugh G. Isolation and characteristics of a semen specific protein from seminal plasma: a potential new marker for semen identification. Journal Forensic Science. 1978;23:106–115
  7. Hochmeister MN, Budowle B, Rudin O, Gehrig C, Borer U, Thali M, et al. Evaluation of Prostate-Specific Antigen (PSA) membrane test assays for the forensic identification of seminal fluid. Journal of Forensic Sciences. 1999;44:1057–1060
  8. Sparkes R, Kimpton C, Watson S, Oldroyd NJ, Clayton T, Barnett L, et al. The validation of a 7-locus multiplex STR test for use in forensic casework (I). International Journal of Legal Medicine. 1996;109:186–194
  9. Sparkes R, Kimpton C, Gilbard S, Carne P, Andersen J, Oldroyd N, et al. The validation of a 7-locus multiplex STR test for use in forensic casework (II). International Journal of Legal Medicine. 1996;109:195–204
  10. Cotton EA, Allsop RF, Guest JL, Frazier RRE, Koumi P, Callow IP, et al. Validation of the Amplifl STR SGMplus system for use in forensic casework. Forensic Science International. 2000;112:151–161
  11. Davis A, Wilson E. The persistence of seminal constituents in the human vagina. Journal of Forensic Sciences. 1974;3:45–55
  12. Reshef A, Barash M, Gallili N, Michael A, Brauner P. The use of acid phosphatase test papers for DNA profiling. Science and Justice. 2005;45(2):97–102
  13. Willott GM, Allard JE. Spermatozoa. Their persistence after sexual intercourse. Forensic Science International. 1982;19:135–154
  14. Keating SM, Allard JE. What's in a name? — Medical samples and scientific evidence in sexual assaults. Medicine, Science and the Law. 1994;34(3):187–201
  15. Allard JE. Collection of data from findings in cases of sexual assault and the significance of spermatozoa on vaginal anal and oral swabs. Science & Justice. 1997;37(2):99–108
  16. Chapman RL, Brown NM, Keating SM. The isolation of spermatozoa from sexual assault swabs using Proteinase K. Journal of the Forensic Science Society. 1989;29:207–212
  17. Gartside BO, Brewer KJ, Strong CL. Estimation of Prostrate-Specific Antigen (PSA) extraction efficiency from forensic samples using Seratec® PSA semiquant Semiquantitative membrane test. Forensic Science Communications. April 2003;5(2):

PII: S1355-0306(07)00098-6

doi: 10.1016/j.scijus.2007.09.010

Science & Justice
Volume 47, Issue 4 , Pages 160-167 , December 2007

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